Monday, August 4, 2014

Algebra "Coin" Problems--5 Simple Rules


Posted by Rebekah and Esther

The dreaded algebra coin problems have come to Planted by Rivers. Okay, okay, so they really shouldn't be dreaded. The truth of the matter is, if you know 5 simple rules, then solving them should be pretty easy.


Here are the rules of the game.

Rule #1:

"Coin "Art by Rebekah
Know how much each coin is worth.

Penny: 1 ¢                                          

Nickel: 5 ¢

Dime: 10 ¢

Quarter: 25 ¢

Half Dollar: 50 ¢


Important note: Because we are working with cents and not dollars, we want to make sure we convert everything in the problem to cents. This will ensure we get rid of any pesky decimals (you’ll see what I mean).


Rule #2: Choose variables (letters) to represent the number of coins.

In other words, if I am working with dimes and quarters, I could use “d” to represent the number of dimes and “q” to represent the number of quarters. It usually helps to use the first letter in the coin’s name (although you could use any letter).


Important note: Don’t forget that each letter represents the NUMBER of coins!


"Coin "Art by Rebekah

Rule #3: Learn what phrases like “4 times as many nickels as dimes” means.

Okay, so to some people this may seem pretty straightforward. First choose a letter to represent the amount of nickels (let’s use “n”), and then choose a letter to represent the amount of dimes (let’s use “d”).

So you should end up with an equation that looks like this: n = 4d

But why not d = 4n? If you’re still mystified, keep on reading! (If not, just skip to rule #4)

Now, let’s start simple. Let’s say we have the phrase “Enoch has 4 times as many balls as Daniel.” So then, if “x” represents the amount of balls Daniel has, Enoch must have 4x balls. So, Enoch’s number of balls = 4x

But what if we say, “Enoch has 4 times as many balls as Daniel has cars”? Well, let’s choose the letter “b” for number of balls and “c” for number of cars. Hmmm…the only real difference between this phrase and the above phrase seems to be that now the concentration is between two objects and not two people. Taking this into consideration, one can see that the correct equation for this problem would be: Enoch’s Number of Balls = 4c or b = 4c.

But look! We can also say: “4 times as many balls as cars,” and the equation still remains b = 4c.

So then, phrases like “4 times as many nickels as dimes,” must mean n = 4d.


Rule #4: Know what “more than” and “less than” mean.

"Coin "Art by Rebekah
Yes, yes, I know—they’re never phrased so nicely. Usually they’ll go something like this: “there are 7 more nickels than pennies” or “there are 7 fewer nickels than pennies”.

Now, let’s use “n” to represent the number of nickels, and “p” to represent the number of pennies. As a result, “there are 7 more nickels than pennies,” must be

n = p + 7 and “there are 7 fewer nickels than pennies” becomes n = p – 7.


WARNING: Although n = p + 7 is the same as n = 7 + p, n = p - 7 is not the same as n = 7 – p. This is because subtraction is not commutative, and as a result, 7 - p could result in a negative number of nickels! 


Rule #5: Do the Algebra!

The best way to explain this is to do actual problems. :) (which I’ll try to post next monday)

To be continued…^_^!



Sunday, August 3, 2014

Science Sunday: DNA Replication (Part 3)--Enzymes

Posted by Rebekah

 Please click here for “DNA Replication (Part 2)

Note: this post describes DNA replication as understood today.

While reading this post, it is important to note that DNA replication in eukaryotes is somewhat different than in prokaryotes (although they do have a lot in common). However, as I mentioned in my last post (yes, it’s been a long time), much of the knowledge we have concerning DNA comes from research involving bacteria. As a result, Lord willing I will be concentrating on DNA replication in E. coli.
OriC Up Close

Origins of DNA Replication

Origins of DNA replication are crucial in that they attract replication enzymes.

But how do they work?

In E. coli, the origin of replication (which is known as oriC) is characterized by an adenine and thymine rich sequence. A-T bonds require less energy to denature than G-C bonds; hence, making it a logical design. This sequence is approximately 245 base pairs (bp) long, and is partitioned by three 13-bp sequences which are then followed by four 9-bp sequences (these are called13-mer and 9-mer sequences respectively).

Replication Enzymes

So, how does it all begin?

An enzyme called DnaA attaches to the 9-mer repeats; The DNA then bends, and the AT-rich 13-mer repeats hydrolyze (break). Thus, resulting in an open complex where the double stranded DNA has begun separating.   

And this is where the drawing I left in my last post comes in handy…



An enzyme called DnaC (not pictured) caries another enzyme called DnaB to oriC. DnaB is a kind of helicase protein, which separates the two complementary stands of DNA by hydrolyzing the hydrogen bonds connecting complementary nucleotides.

This to me (yes, this is my opinion here), reminds me of a zipper: helicase is analogous to a slider and the complementary strands are analogous to the two chains of teeth (See here to learn about the structure of a zipper).

Now, in order to keep the two strands from reannealing (joining again), proteins called single stranded binding proteins (SSB) attach to the unwound strands of DNA.

Unfortunately, all this unwinding causes torsional strain on the DNA; which in turn can lead to supercoiling (kind of like a rubber band when twisted too much). Not surprisingly then, there is another enzyme that relieves this strain—topoisomerase. Topoisomerases do so by catalyzing the cutting and rejoining of the “supercoiled” DNA; hence, causing the DNA to rotate and remove the coil.

But which enzyme is responsible for the synthesis of new DNA daughter strands?

The DNA polymerase III (pol III) holoenzyme!


Note: holoenzymes are enzymes with lots of proteins (as well as other compounds) that help it (the enzyme) do its job.

However, in order to begin work, DNA polymerase needs a 3′-hydroxyl (-OH) group (Lord willing I’ll try covering DNA’s molecular structure later). In order to fix this problem, DnaA, several proteins, and an enzyme called primase unite at oriC and form a complex called the primosome. Primase then synthesizes an RNA primer that provides the very much needed 3′-hydroxyl group. Simply put, an RNA primer is a short stretch of RNA (somewhere between 12 and 24 nucleotides long), and “RNA” (ribonucleic acid) is a lot like DNA, but it uses the nucleotide uracil (U) instead of thymine (T). (Note that there are also several other differences between DNA and RNA not mentioned here).

But how are these RNA primers turned into DNA?

Well, pol III finishes its job once it runs into the RNA primer; subsequently leaving a single-stranded gap between the last DNA nucleotide (of the new daughter strand) and the first RNA nucleotide of the primer. In turn, DNA polymerase III is replaced by an enzyme called DNA polymerase I (pol I), which is attracted to the DNA-RNA single-stranded gap.  DNA polymerase I is special in that it is capable of exonuclease activity. This means it can remove the RNA nucleotides one at a time. Note that, as DNA pol I removes the RNA nucleotides, it replaces them with the necessary DNA nucleotides. All this is done in the 5’ to 3’ direction.

Once the primer has been completely removed, however, there remains a DNA-DNA single-stranded gap. In order to close this gap, an enzyme called DNA ligase steps in, and forms the phosphodiester bond necessary to close this gap.

The Replisome
We have now learned about many of the enzymes associated with DNA replication in E. coli; nonetheless, it would be erroneous to assume that these enzymes act independently from each other. In fact, research now indicates that these proteins and enzymes are all part of larger protein complexes called replisomes.


To be continued… 


                                               Sources
Reece, Jane B., et al. Campbell Biology. 9th Global Edition. “Many Proteins Work Together in DNA Replication and Repair.” Boston: Pearson, 2011. 357-365. Print.
Sanders, Mark F., and John L. Bowman. Genetic Analysis: An Integrated Approach. 1st ed. N.p.: Benjamin Cummings, 2012. Print.

WARNING: Due to several reasons, I do NOT recommend Campbell Biology for your homeschool. However, due largely impart to its prevalent use in colleges and universities (and even Wikipedia), I chose to use it as a reference.  
                                                         
                                                                      

Tuesday, April 8, 2014

More Birthday Cake Pictures--and Hopefully More Posting



Posted by Esther


Wow, we haven't posted here for over eight months now; however, we're planning on changing that soon! Lord willing, we want to revive this blog by posting an array of topics that will hopefully, be of interest to readers. We're also planning on posting more often, as that has been a struggle ever since we started this blog.

Well for now I decided to post some pictures of some birthday cakes I made recently. As I mentioned earlier in another post, we like to make our cakes from scratch at our home; and since I love to bake and cake-decorate, I'm usually the one to make my siblings' birthday cakes.

Here are the pictures (they're from 2013-2014):




This red guy--I'm not sure what kind of creature he is--is from a computer game my brother (or should I say brothers) likes to play.

             This cake was for my youngest sister's first birthday--which we recently celebrated!


This is a penguin from a movie my younger siblings love--his name is Scamper (just in case anyone is curious ;) ). His beak was made out of a carrot that was covered in orange frosting, and the top of his hat was simply a piece of curly-leaf lettuce--strange, I know :) . Oh, and his eyes and eyebrows were made of raisins.

 

I'll leave you all with this famous piece of scripture from Matthew, that I was just recently studying; although it has nothing to do with what I posted above, I find it too beautiful not to post.  

{Emphasis is mine}.

Now when the tempter came to Him, he said, “If You are the Son of God, command that these stones become bread.” But He answered and said, “It is written, ‘Man shall not live by bread alone, but by every word that proceeds from the mouth of God."


Matthew 4:3-4
 

Well, good bye for now!





Sunday, August 11, 2013

Science Sunday: DNA Replication (Part 2)



Posted by Rebekah

 Please click here for “DNA Replication (Part 1).”



Last time I said that DNA replication in prokaryotes is different from that in eukaryotes. DNA replication in the bacteria Escherichia coli (i.e. E. coli) is often used to exemplify these differences.

Below I briefly summarize these differences.  


First, it is important to note that the E. coli chromosome is circular and only has one origin of replication—this is the case for most other bacteria.   



As expected, replication begins at the origin of replication; hence, forming a replication bubble and two replication forks. Please note that the daughter strand is shown in light green.


Finally, two new daughter DNA molecules are formed. 

DNA Replication—a Closer Look
 
A closer look at DNA replication
In order to facilitate this section, I have made a drawing (see above) of only some of the many enzymes and proteins involved in DNA replication. Please note that, as implied in the previous post, DNA synthesis is no easy feat. Not surprisingly, one finds that there is still much to learn about this astounding process.

Much of the current knowledge of DNA replication comes from bacterial DNA, so my goal is to concentrate primarily on bacteria. Please study the drawing above carefully (which, by the way is conveniently color coded), as it will come in handy for subsequent posts.     
 
To be continued...

                                                            References 

Reece, Jane B., et al. Campbell Biology. 9th Global Edition. “Many Proteins Work Together in DNA Replication and Repair.” Boston: Pearson, 2011. 357-365. Print.


WARNING: Due to several reasons, I do NOT recommend Campbell Biology for your homeschool. However, due largely impart to its prevalent use in colleges and universities (and even Wikipedia), I chose to use it as a reference.